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Incorporation allows you to protect:

You’ve worked hard to build your brand and image. By incorporating, you can protect it from being used by others for their benefit.

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You might not be a hundred percent clear on what perpetual existence means , but it sounds good, right? Legally, by incorporating, you protect your business forever. It is important to know, of course, that your business can still be sold or that you may close it at some point. However, within the definition of a corporation is the business’ ability to remain in perpetual existence as its own entity. No matter what happens to those who are involved in the business, the business can remain operational and profitable.

Here are just a few of the many reasons this matters:

That being said, perpetual existence becomes a powerful and necessary tool for any business that wants to establish a strong foundation from which to grow.

Here’s one of the benefits of incorporating many people often miss. Let’s say that you want to pass your business on to your son or daughter as you get older, but only want to do so in the event of a sudden illness. It is easier to transfer ownership and funds when the business is a corporation than it is if you are running a sole proprietorship. Remember, in a sole proprietorship, all of your personal assets are linked to your business. It is not possible to easily value your business for a sale, or transfer it to another person until each of these lines of connections to your personal assets are defined and cut.

Whether for short term or long term goals, your business will benefit significantly from incorporating for this reason alone. There are some restrictions of course, but transferring funds and even business ownership is easier when the business has its own identity.

Opening a local shop may be your passion and goal. But, ultimately, all business owners want their business to go on long after they are gone. If you own a sole proprietorship and you pass away, the business passes to your heirs. Your heirs are those named in your will or those who are your next of kin (your spouse, your children, etc). This happens regardless of their desire to own the business. However, this is another of the hidden benefits of incorporating.

When your business is a corporation, even though you still run it, it is its own entity. It continues to exist even after you are gone as if nothing has changed. Your business will likely need a new head, but the business doesn’t halt.

You might be wondering if this still applies to those who plan on leaving their business to their heirs . The simple answer is yes. When a person dies, especially without a trust protecting the business, the entire estate (which would include your business) goes through a probate court. Depending on your state’s laws, high taxation can occur on that estate. The value of the estate is used to repay debts, including any mortgages, loans, or medical bills you might have left after passing. Only after all of this happens does whatever is remaining pass to your heirs. That could be much less than the value of your business.

We then examined the nature of the glycosyl chain more closely by enzymatic digestion. Endoglycosidase H (EndoH) cleaves all N-linked carbohydrate chains normally occurring in the ER between the two innermost GlcNAc residues, leading to very fast migration of the digested product. EndoH digestion was used to confirm that the isolated glycotripeptides contain typical N-linked carbohydrate chains ( Fig. 9C , pancreas and adrenal, lane E). In contrast, Jack bean α-mannosidase is an exomannosidase that cleaves only terminal α-linked mannoses. In dog pancreas-derived glycotripeptides, incubation with this enzyme led to a complete digestion down to the GlcNAc 2 βMan core ( Fig. 9C , pancreas, lane M). In contrast, when glycotripeptides derived from adrenal smooth microsomes were subjected to this digest ( Fig. 9C , adrenal, lane M), an additional spot with lower Rf value was seen. This partially resistant material presumably retains one or more of the terminal glucose residues that are initially present on the transferred N-glycosyl chain (Glc 3 Man 9 GlcNAc 2 ). This assumption was confirmed by comparison with glycotripeptides generated in the presence of castanospermine, a glucosidase inhibitor (data not shown). Thus, the slower migration of adrenal-derived material can be explained by a low activity of at least one glucosidase (I and/or II) in adrenal microsomes. If glucosidase II were preferentially affected, retention of monoglucosyl residues could result in more extended association with chaperones such as calnexin, abundant in the smooth microsomes, facilitating retention of glycosylated proteins in the SER ( 56 , 57 ).

Having found that the OST components formed a functional unit in adrenal smooth microsomes, we then sought to determine whether the SR and Sec61 complexes function in this setting. To determine SR activity, we took advantage of a previously developed photocross-linking assay in which one can assess SR activity by looking for a decrease in the amount of signal peptide-associated SRP54 ( 23 ). Truncated RNCs for 86pPL containing photocross-linker-modified lysines, were prepared in a reticulocyte lysate system. In absence of microsomal membranes a 62-kDa photoadduct formed representing the 86pPL signal peptide cross-linked to SRP 54 kDa ( 24 , 25 ). Addition of SR-containing dog rough microsomes reduces the intensity of the SRP cross-link due to the release of SRP from the nascent chain by SR present in the microsomal membranes ( 23 ). In this assay, adrenal smooth microsomes were capable of decreasing the intensity of the SRP cross-link, although not to the same extent as dog pancreatic microsomes ( Fig. 10A ). Because the levels of SRα are lower in adrenal smooth microsomes than in liver or pancreas rough microsomes, it is possible that some ribosome-nascent chain-SRP complexes interacted with SR-deficient Sec61 complexes and were therefore unable to release SRP.

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